Narayanan Parameswaran, BVSc., Ph.D.
Our lab is interested in the area of innate immunity, inflammation and cell signaling in the context of gut and bone pathophysiology. Part of our efforts is focused on identifying the immunoregulatory mechanisms underlying inflammatory diseases (including sepsis and inflammatory bowel disease), particularly those related to GPCR regulators arrestins and G-protein coupled receptor kinases. In addition, we work closely with Dr. McCabe’s lab in establishing mechanisms that regulate gut-bone signaling. In this context, our labs have used a variety of approaches including probiotics to modulate gut-bone axis.
Complete List of Published Work:
Following are the general outlines of the projects that are currently in progress:
- ARRESTINS IN INFLAMMATORY SIGNALING AND ROLE IN SEPSIS AND COLITIS: One of the research areas that our lab has focused on is to understand the role of arrestins in inflammatory diseases. Arrestins are intracellular scaffolding proteins discovered for their role in GPCR desensitization. Work during my postdoctoral training and research from my lab have uncovered several novel functions of arrestins in inflammatory diseases particularly relevant to sepsis and colitis. An emerging theme that came out of studies from our lab and others is that arrestin function in inflammatory disease is highly context dependent. Depending on the stimulus and/or disease, arrestins can either mediate or inhibit inflammation. Some of the major findings from our studies include the discovery that arrestin-2 and arrestin-3 are important regulators of signaling from Toll-like receptor (non-GPCRs) and that they play distinct and overlapping roles in inflammatory diseases (sepsis versus colitis); More recently, our studies also demonstrate that the hematopoietic- and non-hematopoietic-cell specific arrestin-2 oppose each other mechanistically in the pathogenesis of inflammatory disease.
- Lee T, Lee E, Arrollo D, Lucas PC, Parameswaran N. Non-Hematopoietic β-Arrestin1 Confers Protection Against Experimental Colitis. J Cell Physiol. 2016 May;231(5):992-1000. PubMed PMID: 26479868; PubMed Central PMCID: PMC4728047.
- Sharma D, Parameswaran N. Multifaceted role of β-arrestins in inflammation and disease. Genes Immun. 2015 Dec;16(8):576. PubMed PMID: 26633148.
- Sharma D, Malik A, Steury MD, Lucas PC, Parameswaran N. Protective Role of β-arrestin2 in Colitis Through Modulation of T-cell Activation. Inflamm Bowel Dis. 2015 Dec;21(12):2766-77. PubMed PMID: 26296063; PubMed Central PMCID: PMC4654649.
- Sharma D, Packiriswamy N, Malik A, Lucas PC, Parameswaran N. Nonhematopoietic β-Arrestin-1 inhibits inflammation in a murine model of polymicrobial sepsis. Am J Pathol. 2014 Aug;184(8):2297-309. PubMed PMID: 24946011; PubMed Central PMCID: PMC4116700.
- REGULATION OF INFLAMMATORY SIGNALING AND DISEASE BY G-PROTEIN COUPLED RECEPTOR KINASES (GRKs): GRKs were originally discovered for their role in G-protein coupled receptor desensitization. Our lab has been interested in understanding the non-canonical roles of GRKs especially in the immune system and found that GRKs are important regulators of NFkappaB signaling. In addition, we have made some important observations including the role of GRK5 in sepsis and GRK2 in inflammatory bowel disease. Overall our studies suggest that GRKs could be therapeutic targets in the treatment of inflammatory diseases and are currently pursuing collaborative avenues to target these kinases in inflammatory diseases.
- Steury MD, Kang HJ, Lee T, Lucas PC, McCabe LR, Parameswaran N. G protein-coupled receptor kinase-2-deficient mice are protected from dextran sodium sulfate-induced acute colitis. Physiol Genomics. 2018 Jun 1;50(6):407-415. PubMed PMID: 29570431.
- Steury MD, McCabe LR, Parameswaran N. G Protein-Coupled Receptor Kinases in the Inflammatory Response and Signaling. Adv Immunol. 2017;136:227-277. PubMed PMID: 28950947; PubMed Central PMCID: PMC5730335.
- Packiriswamy N, Lee T, Raghavendra PB, Durairaj H, Wang H, Parameswaran N. G-protein-coupled receptor kinase-5 mediates inflammation but does not regulate cellular infiltration or bacterial load in a polymicrobial sepsis model in mice. J Innate Immun. 2013;5(4):401-13. PubMed PMID: 23485819; PubMed Central PMCID: PMC3753665.
- Patial S, Luo J, Porter KJ, Benovic JL, Parameswaran N. G-protein-coupled-receptor kinases mediate TNFα-induced NFκB signalling via direct interaction with and phosphorylation of IκBα. Biochem J. 2009 Dec 14;425(1):169-78. PubMed PMID: 19796012; PubMed Central PMCID: PMC2856098.
- MECHANISTIC BASIS OF GUT-BONE SIGNALING: In an effort to understand how gut as an organ can influence other organs in the body, we established collaborations with Drs. McCabe (a bone and gut physiologist) and Britton (a Microbial geneticist) to modulate gut environment to test the effects on bone. The initial observations by Drs. McCabe and Britton set out the fundamental idea that oral treatment of probiotics can increase bone in mice. This quickly evolved into testing the effects of probiotics in clinically relevant models of bone loss including menopause and diabetes. Through collaborative efforts of the three labs (Drs. McCabe, Britton and Parameswaran), we identified that oral treatment of mice with probiotics in estrogen deficient mice (menopause model) not only prevents bone loss, but also alters bone marrow immune cells in a favorable manner. We are currently testing how estrogen deficiency and diabetes induces gut and bone inflammation, whether they are linked, and if so, whether probiotics are acting at the level of gut inflammation and/or bone inflammation.
- McCabe LR, Parameswaran N. Advances in Probiotic Regulation of Bone and Mineral Metabolism. Calcif Tissue Int. 2018 Apr;102(4):480-488. PubMed PMID: 29453726; PubMed Central PMCID: PMC5849527.
- McCabe LR, Irwin R, Tekalur A, Evans C, Schepper JD, Parameswaran N, Ciancio M. Exercise prevents high fat diet-induced bone loss, marrow adiposity and dysbiosis in male mice. Bone. 2018 Mar 29;PubMed PMID: 29604350.
- Quach D, Collins F, Parameswaran N, McCabe L, Britton RA. Microbiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice. mSphere. 2018 Jan-Feb;3(1)PubMed PMID: 29299532; PubMed Central PMCID: PMC5750390.
- Collins FL, Schepper JD, Rios-Arce ND, Steury MD, Kang HJ, Mallin H, Schoenherr D, Camfield G, Chishti S, McCabe LR, Parameswaran N. Immunology of Gut-Bone Signaling. Adv Exp Med Biol. 2017;1033:59-94. PubMed PMID: 29101652; PubMed Central PMCID: PMC5749247.
- Britton RA, Irwin R, Quach D, Schaefer L, Zhang J, Lee T, Parameswaran N, McCabe LR. Probiotic L. reuteri treatment prevents bone loss in a menopausal ovariectomized mouse model. J Cell Physiol. 2014 Nov;229(11):1822-30. PubMed PMID: 24677054; PubMed Central PMCID: PMC4129456.