Robert Wiseman, Ph.D.
Ph.D., Florida State University
Metabolic Regulation in Excitable Cells: Skeletal Muscle
Muscle Blood Flow
We study the regulation of metabolism in excitable cells including skeletal muscle, brain and heart. Our particular focus is on skeletal muscle because its physiologic function is well defined, easily measured and it dictates the metabolic responses through the demand for ATP. Our musculature is the largest organ system in the body comprising 40-45% of body mass in the average adult and is important in glucose homeostasis. Furthermore the extensive benefits of exercise are well documented. Understanding metabolic flux, substrate utilization and mitochondrial function are critical to resolve the pathophysiology associated with metabolic diseases such as diabetes and metabolic syndrome. Our laboratory uses magnetic resonance imaging and spectroscopy to non-invasively measure blood flow, perfusion and energy dynamics respectively. We also use a host of computational and biophysical techniques from mathematical modeling to high resolution respirometry, genetic modifications in transgenic animals and viral vectors.
- Lemaster KA, Frisbee SJ, Dubois L, Tzemos N, Wu F, Lewis MT, Wiseman RW, Frisbee JC. 2018. Chronic atorvastatin and exercise can partially reverse established skeletal muscle microvasculopathy in metabolic syndrome. Am J Physiol-Heart Circ Physiol 315: H855–H870.
- Frisbee JC, Lewis MT, Wiseman RW. 2018. Skeletal muscle performance in metabolic disease: microvascular or mitochondrial limitation or both? Microcirc. N. Y. N 1994. doi: 10.1111/micc.12517.
- Frisbee JC, Lewis MT, Kasper JD, Chantler PD, Wiseman RW. 2018. Type II diabetes mellitus in the Goto-Kakizaki rat impairs microvascular function and contributes to premature skeletal muscle fatigue. J. Appl. Physiol. doi:10.1152/japplphysiol.00751.2018.
- Kasper JD, Meyer RA, Beard DA, Wiseman RW. 2019. Effects of altered pyruvate dehydrogenase activity on contracting skeletal muscle bioenergetics. Am J Physiol Regul Integr Comp Physiol. 2019 Jan 1;316(1):R76-R86. doi: 10.1152/ajpregu.00321.2018. Epub 2018 Nov 21.
- Lewis MT, Lujan HL, Tonson A, Wiseman RW, DiCarlo SE. 2019. Obesity and inactivity, not hyperglycemia, cause exercise intolerance in individuals with type 2 diabetes: Solving the obesity and inactivity versus hyperglycemia causality dilemma. Med Hypotheses 123: 110–114.
- Diaz-Otero JM, Yen T, Ahmad A, Laimon-Thomson E, Abolibdeh B, Kelly K, Lewis MT, Wiseman RW, Jackson WF, and Dorrance AM. Transient receptor potential vanilloid 4 channels are important regulators of parenchymal arteriole dilation and cognitive function. Microcirculation. (In press).