Kathleen Gallo, Ph.D.
Areas of Research Interest
The Gallo laboratory is interested in the understanding and therapeutic targeting of signaling pathways that govern cancer progression. In particular, we have focused on the mixed-lineage kinase (MLK) family and their roles in cancer invasion and metastasis, particularly in the context of breast cancer. We have demonstrated a critical role for MLK3 in triple-negative breast cancer metastasis in regulating both focal adhesion turnover during cell migration and gene expression during invasion and metastasis. In addition we are studying the roles of MLK family members and related signaling in lung cancer, glioblastoma, and melanoma.
Our laboratory uses diverse approaches and techniques to study mechanisms of tumor proliferation, invasion and metastatic outgrowth including 3D organotypic assays, cell biology, animal models, mass spectrometry, and biochemical techniques.
We collaborate with the Conrad lab (Microbiology & Molecular Genetics) in identifying mechanisms and alternative therapeutic strategies for endocrine resistant breast cancer. In collaboration with the Neubig lab in Pharmacology & Toxicology, we are exploring appropriate combination therapies in drug-resistant melanoma.
MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells. Rattanasinchai C, Llewellyn BJ, Conrad SE, Gallo KA. Oncogenesis. 2017 Jun 12;6(6):e345. doi: 10.1038/oncsis.2017.44. PMID: 28604765
EGFR Signals through a DOCK180-MLK3 Axis to Drive Glioblastoma Cell Invasion. Misek SA, Chen J, Schroeder L, Rattanasinchai C, Sample A, Sarkaria JN, Gallo KA. Mol Cancer Res. 2017 May 9. doi: 10.1158/1541-7786.MCR-16-0318. [Epub ahead of print] PMID:28487380
MLK3 Signaling in Cancer Invasion. Rattanasinchai C, Gallo KA. Cancers (Basel). 2016 May 19;8(5). pii: E51. doi: 10.3390/cancers8050051. Review. PMID: 27213454
Tamkus D, Sikorskii A, Gallo K.A., Wiese DA, Leece C, Madhukar BV, Chivu SC, Chitneni S, and Dimitrov NV. Endothelin-1 Enriched Tumor Phenotype Predicts Breast Cancer Recurrence, ISRN Oncology. 2013, Article ID 38539.
Wang L, Gallo K.A., Conrad SE. Targeting mixed lineage kinases in ER-positive breast cancer cells leads to G2/M cell cycle arrest and apoptosis Oncotarget, 2013. 4(8).
Wu X, Gallo K.A. The 18-kDa translocator protein (TSPO) disrupts mammary epithelial morphogenesis and promotes breast cancer cell migration PLOS ONE. 2013, 8:e71258.
Chen J, Rattanasinchai C, Gallo K.A., (2012) “Mixed Lineage Kinase 3” Encyclopedia of Signaling Molecules, Springer Press, Sangdun Choi, Ed.
Chen J, and Gallo K.A., MLK3 regulates paxillin phosphorylation in chemokine-mediated breast cancer cell migration and invasion to drive metastasis. Cancer Research. 2012, 72: 4130-40.
Kovalenko PL, Kunovska L, Chen J, Gallo K.A., Basson MD. Loss of MLK3 signaling impedes ulcer healing by modulating MAPK signaling in mouse intestinal mucosa. Am J Physiol Gastrointest Liver Physiol. 2012, 303:G951-60.
Liou GY, Zhang H, Miller EM, Seibold SA, Chen W, Gallo K.A., Induced, selective proteolysis of MLK3 negatively regulates MLK3/JNK signaling. Biochem J. 2010, 427:435-43.
Chen J, Miller EM and Gallo K.A., MLK3 is critical for breast cancer cell migration and promotes a malignant phenotype in mammary epithelial cells. Oncogene. 2010, 29: 4399-411.